Discussion
A microarray differs from a gene fusion in that, it
Answer: D
Correct answer: all of the above
All three statements hold. A microarray carries DNA segments from many different genes - thousands of spots, potentially a whole genome - whereas a gene fusion reports on a single promoter. It is not built by cloning but by spotting or synthesising oligonucleotides directly on a solid support. And by hybridising labelled cDNA to those spots it measures transcript abundance directly.
A gene fusion does the opposite on each count: one promoter, constructed by cloning, and read out indirectly through the activity of a reporter protein such as beta-galactosidase. The two techniques are complementary, one broad and one deep.
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