Discussion
Which of the following type of recombination does not require homologous sequences and is important for the integration of viral genomes into bacterial chromosomes?
Answer: B
Correct answer: Site-specific recombination
Site-specific recombination requires no general homology. It acts at short, defined sequences recognised by a dedicated integrase, which is exactly how the lambda phage genome enters the bacterial chromosome: the phage attP site and the bacterial attB site share only a short core sequence, and integrase catalyses the exchange between them.
General recombination, by contrast, depends on extensive homology and RecA. Replicative recombination is the mechanism of transposition, and mutagenic recombination is not the standard name for phage integration.
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