Discussion
When composite transposons are formed
Answer: D
Correct answer: two IS elements integrate into a chromosome with only a small distance separating them
A composite transposon is formed when two insertion sequences land close together and the DNA between them becomes mobile as a unit. The transposases act on the outermost inverted repeats, so everything lying between the two IS elements - commonly antibiotic resistance genes - is cut out and moved along with them.
Tn5 and Tn10 are built exactly this way, which is how resistance genes acquire mobility they did not originally have. No deletion in the transposase gene is required, and no plasmid is needed for the structure to form.
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