Discussion
The expression of gene X (which has promoter Px) is to be monitored. A gene fusion construction for carrying this work will
Answer: A
Correct answer: have Px but not the rest of the X coding region
A gene fusion built to monitor gene X joins the promoter Px to a reporter coding sequence such as lacZ. The construct therefore keeps Px, which carries the regulatory information being studied, and discards the X coding region, whose product is not what is being measured.
It must not carry the reporter's own promoter, or the reporter would be transcribed independently of Px and report nothing about gene X. The construct reports on this one promoter, not on every promoter of similar sequence, and it measures promoter activity indirectly through enzyme output rather than giving the direct transcript measurement a microarray provides.
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