Discussion
Which of the following statement(s) is/are true in regards to F+ x F- mating events?
Answer: B
Correct answer: DNA is transferred from F+ to F- cells
In an F+ × F− mating the DNA moves from donor to recipient - from F+ to F−. The F+ cell makes the sex pilus, contact is established, and one strand of the F plasmid is nicked at oriT and transferred; both cells then synthesise the complementary strand, so the recipient becomes F+.
Transfer is therefore one-way, defined by which cell carries the F factor. The F− cell is not incapable of conjugating - it is a perfectly good recipient - it simply lacks the plasmid that encodes the machinery, so it cannot act as donor.
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