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Transduction is the transfer of bacterial DNA by

  • A.a bacteriophage
  • B.direct cell contact
  • C.free DNA in the medium
  • D.a plasmid alone

Answer: C

Correct answer: a bacteriophage

Transduction is the transfer of bacterial DNA from one cell to another by a bacteriophage, discovered by Zinder and Lederberg in 1952. It occurs in two forms. In generalised transduction, a phage in the lytic cycle packages a fragment of host DNA into a capsid by mistake, so any gene may be carried; the resulting particle injects bacterial rather than phage DNA into the next cell. In specialised transduction, a temperate phage integrated as a prophage excises imprecisely and carries the adjacent host genes with it - so only genes flanking the integration site can be transferred, which for phage lambda in E. coli means the gal and bio genes. The distinction is worth holding: generalised transduction moves any gene at random, specialised transduction moves particular genes only. It is used experimentally for fine-scale genetic mapping and for moving mutations between strains.

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