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The great plate count anomaly refers to the observation that

  • A.Different growth media give very nearly the same count
  • B.Far fewer organisms grow on plates than are seen under the microscope
  • C.Plates consistently overestimate the population actually present
  • D.Colony diameters vary widely between replicate plates of one sample

Answer: B

Direct microscopic counts of soil routinely exceed plate counts by two or three orders of magnitude, because the great majority of soil organisms will not grow on standard laboratory media. It is the central reason molecular methods displaced cultivation for community surveys.

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