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The most probable number technique estimates population size from

  • A.The turbidity of a single culture read against a calibration standard
  • B.The dry weight of cells harvested by centrifugation
  • C.The pattern of growth and no growth across replicate dilution tubes
  • D.The diameter reached by colonies on a spread agar plate

Answer: C

A set of tubes is inoculated at several dilutions and scored simply for growth or no growth, and the pattern of positives is referred to a statistical table. It suits organisms that are awkward to count on plates, such as nitrifiers, which grow slowly and form no visible colony.

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