Discussion
Which of the following best describes principle of western blotting technique ?
Answer: D
Western blotting is an immunoassay technique preceded by electrophoresis.
Proteins are first separated by size on an SDS-polyacrylamide gel, then transferred (blotted) onto a nitrocellulose or PVDF membrane. A specific primary antibody detects the target protein, and a labelled secondary antibody reveals it.
Separating restriction fragments by size describes Southern blotting of DNA. Attraction to a specific chemical group describes affinity chromatography. Zero net charge at a particular pH is the isoelectric point, the principle of isoelectric focusing.
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