Discussion
Select the technique most appropriate to demonstrate that lactose induces the synthesis of β-galactosidase enzyme.
Answer: B
The most appropriate technique is the Western Blot.
The claim is about synthesis of the enzyme, a protein. A Western blot separates cell proteins by electrophoresis and detects β-galactosidase with a specific antibody, so comparing cells grown with and without lactose shows directly whether the protein appears on induction.
A Northern blot or quantitative PCR (on cDNA) would show an increase in lacZ mRNA, which demonstrates transcription rather than synthesis of the protein. A Southern blot detects DNA, and the gene is present whether or not it is induced.
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