Discussion
With reference to virulence (vir) region of nopaline type Ti plasmid of Agrobacterium tumefaciens, match Group-I (vir gene) and Group-II (coded protein) in CORRECT combination.
| Group-I | Group-II |
|---|---|
| P. vir A | I. Single strand T-DNA binding protein |
| Q. vir B | II. Topoisomerase |
| R. vir E | III. Membrane protein, channel for T-DNA |
| S. vir D | IV. Sensor protein, constitutive expression |
Answer: B
The correct combination is P-IV, Q-III, R-I, S-II.
- vir A encodes the membrane sensor kinase that detects plant phenolics such as acetosyringone; it is expressed constitutively at a low level, so it can sense the wound signal (IV).
- vir B encodes the proteins of the type IV secretion apparatus, the membrane channel through which the T-strand leaves the bacterium (III).
- vir E encodes VirE2, the single-strand DNA binding protein that coats the T-strand in the plant cell (I).
- vir D encodes VirD1, which has topoisomerase-like activity, and VirD2, the endonuclease that nicks the border repeats (II).
Option A swaps vir E and vir D, option C misplaces vir B, and option D puts DNA binding on the sensor gene vir A.
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