Discussion
Match the tasks given in Group I with the associated techniques conventionally used as listed in Group II.
| Group I | Group II |
|---|---|
| P. Ploidy analysis | 1. RNA sequencing |
| Q. Profiling DNA methylation | 2. Exome sequencing |
| R. Identifying non-coding RNAs | 3. Fluorescence in situ hybridization |
| S. Identifying SNPs | 4. Bisulfite sequencing |
| T. Satellite DNA isolation | 5. Density-gradient centrifugation |
Answer: B
The match is P-3; Q-4; R-1; S-2; T-5.
- Ploidy can be counted with fluorescence in situ hybridization, by the number of signals a chromosome-specific probe gives in each nucleus.
- Bisulfite sequencing converts unmethylated cytosines to uracil while methylated ones remain, so it profiles DNA methylation.
- RNA sequencing reads the whole transcriptome, including non-coding RNAs.
- Exome sequencing reads all exons, a standard way to identify single nucleotide polymorphisms in genes.
- Satellite DNA has a different base composition and so forms separate 'satellite' bands in density-gradient centrifugation.
The other options misassign at least one technique, for example using density-gradient centrifugation for methylation profiling.
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