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Discussion

Match the tasks given in Group I with the associated techniques conventionally used as listed in Group II.

Group IGroup II
P. Ploidy analysis1. RNA sequencing
Q. Profiling DNA methylation2. Exome sequencing
R. Identifying non-coding RNAs3. Fluorescence in situ hybridization
S. Identifying SNPs4. Bisulfite sequencing
T. Satellite DNA isolation5. Density-gradient centrifugation

  • A.P-2; Q-1; R-3; S-4; T-5
  • B.P-3; Q-4; R-1; S-2; T-5
  • C.P-5; Q-4; R-1; S-2; T-3
  • D.P-3; Q-5; R-1; S-2; T-4

Answer: B

The match is P-3; Q-4; R-1; S-2; T-5.

  • Ploidy can be counted with fluorescence in situ hybridization, by the number of signals a chromosome-specific probe gives in each nucleus.
  • Bisulfite sequencing converts unmethylated cytosines to uracil while methylated ones remain, so it profiles DNA methylation.
  • RNA sequencing reads the whole transcriptome, including non-coding RNAs.
  • Exome sequencing reads all exons, a standard way to identify single nucleotide polymorphisms in genes.
  • Satellite DNA has a different base composition and so forms separate 'satellite' bands in density-gradient centrifugation.

The other options misassign at least one technique, for example using density-gradient centrifugation for methylation profiling.

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