Discussion
Which of the following selectable marker genes is/are used for herbicide tolerance during genetic transformation of plants?
Answer: B
The herbicide tolerance marker is bar.
The bar gene from Streptomyces hygroscopicus encodes phosphinothricin acetyltransferase, which inactivates the herbicide phosphinothricin (glufosinate, the active ingredient of Basta). Transformed plant cells survive on media or sprays containing it.
hpt confers resistance to the antibiotic hygromycin and nptII to kanamycin, so both are antibiotic markers. pmi (phosphomannose isomerase) is a positive selection marker that lets cells grow on mannose. Of the four, only bar applies, so this multiple-select question has a single correct choice.
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