Discussion
Förster Resonance Energy Transfer does NOT depend on the
Answer: B
FRET does not depend on the fluorescence quantum yield of the acceptor.
The efficiency of Förster transfer is set by the distance r between donor and acceptor relative to the Förster radius \(R_{0}\), where
\[ R_{0}^{6} \propto \kappa^{2}\,\Phi_{D}\,J\,n^{-4} \]
Here \(\kappa^{2}\) is the orientation factor, \(\Phi_{D}\) the quantum yield of the donor, J the spectral overlap of donor emission with acceptor absorption, and n the refractive index. The efficiency itself falls as \(r^{-6}\).
The acceptor only has to absorb the energy; transfer happens whether or not it then fluoresces (a non-fluorescent quencher works as an acceptor). Orientation, distance and spectral overlap all appear in the expression, so they cannot be the answer.
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