Discussion
Correctly match the enzymes used as selectable markers (Group I) and the chemicals used for their selection (Group II).
| Group I | Group II |
|---|---|
| (P) Neomycin phosphotransferase | (1) Bialaphos |
| (Q) Phosphinothricin acetyltransferase | (2) Kanamycin |
| (R) Dihydrofolate reductase | (3) Glyphosate |
| (S) 5-Enolpyruvyl shikimate 3-phosphate synthase | (4) Methotrexate |
Answer: A
The correct match is P-2; Q-1; R-4; S-3.
- Neomycin phosphotransferase (nptII) phosphorylates aminoglycosides, so transformed cells survive kanamycin.
- Phosphinothricin acetyltransferase (bar/pat) acetylates phosphinothricin, the active part of the herbicide bialaphos.
- Dihydrofolate reductase, in a resistant form, lets cells grow on methotrexate, an inhibitor of the normal enzyme.
- EPSP synthase, in a glyphosate-insensitive form, confers resistance to glyphosate, which inhibits the native enzyme.
The other options misplace these pairs, for example giving neomycin phosphotransferase bialaphos or pairing DHFR with glyphosate, whose target is EPSPS.
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