Discussion

Correctly match the following molecules/methods in Group-I with their major uses in Group-II.

Group-I	Group-II
(P) CRISPR-Cas9	(1) Global gene expression analysis
(Q) DNA Microarray	(2) Molecular marker
(R) Vir genes	(3) Targeted gene editing
(S) RAPD	(4) Agrobacterium-mediated plant transformation

Answer: B

The correct matching is P-3; Q-1; R-4; S-2.

CRISPR-Cas9 uses a guide RNA to bring the Cas9 nuclease to a chosen DNA sequence: targeted gene editing (3).
A DNA microarray measures the hybridisation of thousands of transcripts at once: global gene expression analysis (1).
The vir genes of the Ti plasmid process and transfer T-DNA into the plant cell, the basis of Agrobacterium-mediated transformation (4).
RAPD (random amplified polymorphic DNA) uses short random primers to reveal polymorphic bands, a molecular marker (2).

The options that give CRISPR-Cas9 the expression-analysis role or put the vir genes down as a marker swap these well-defined uses.

No comment is present. Be the first to comment.

Loading…