Discussion
Which of the following statements is/are NOT correct about the technique used in molecular biology?
Answer: D
The statement that is NOT correct is ELISA is used to detect specific DNA sequences directly without antibodies; it is the only one the key marks in this multiple-select question.
ELISA, the enzyme-linked immunosorbent assay, depends on antibodies by definition: an antibody (or antigen) is fixed to a plate, the target binds, and an enzyme-linked antibody produces a coloured or fluorescent signal. It is normally used to detect and quantify proteins and other antigens, not DNA sequences.
The other three statements are correct: Southern blotting detects specific DNA sequences with a labelled probe, Northern blotting detects RNA and so reports expression levels, and Western blotting detects proteins with antibodies.
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