Discussion
Which of the following technique is best for determining the precise location of the radioactive isotope located in a specimen ?
Answer: C
The best technique is autoradiography.
In autoradiography the specimen, a tissue section, gel or blot, is placed against photographic emulsion or film. Radiation from the isotope exposes the emulsion just where the labelled molecules are, giving an image of their precise location. It is how pulse-chase experiments traced newly made proteins through the ER and Golgi.
Ultracentrifugation separates particles by size and density but does not show location in a specimen. Atomic force microscopy maps surface topography, and fluorescence microscopy detects fluorophores, not radioactive decay.
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