Discussion
The protein binding site in DNA can be identified by the following experiment :
Answer: A
The protein binding site is identified by DNA footprinting.
In footprinting, a DNA fragment labelled at one end is lightly digested with DNase I (or cut with hydroxyl radicals) with and without the bound protein. The protein shields the bases it covers, so on a sequencing gel the ladder of fragments shows a gap, the 'footprint', exactly at the binding site, which can be read against a sequence ladder.
A mobility shift assay (EMSA) shows that a protein binds a DNA fragment, by slowing its migration, but not where on the fragment. Western blotting detects proteins with antibodies, and DNA fingerprinting identifies individuals from repeat polymorphisms.
Post your comment
Please log in or register to post a comment.
Examination Categories
- SSC Exams
- Banking Exams
- Teaching Exams
- Civil Services Exams
- Railways Exams
- Engineering Recruitment Exams
- Defence Exams
- State Government Exams
- Police Exams
- Insurance Exams
- Nursing Exams
- Other Government Exams
- NRA CET
- PG Entrance Exams
- Campus Placement
- MBA Entrance Exams
- CUET and UG Entrance
- Judiciary Exams
- Regulatory Body Exams
- Software Development