Discussion
Three of the following are two dimensional experiments. Which is the odd one out?
DEPT
COSY
HSQC
NOESY
Answer: A
COSY, HSQC and NOESY all build a second frequency dimension by incrementing an evolution period, whereas DEPT is a one dimensional experiment that uses a variable final pulse angle to edit the carbon spectrum by attached proton count. Its output is a single spectrum with peaks pointing up or down, not a contour map. The distinction matters when planning instrument time, since DEPT takes minutes and a good HMBC can take hours.
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