Discussion
Which one of the following compounds does NOT block electron transport?
Answer: C
The compound that does not block electron transport is Oligomycin.
Oligomycin inhibits ATP synthase by binding to its Fo proton channel. It blocks phosphorylation, not the electron transport chain itself; in tightly coupled mitochondria electron flow slows only indirectly, because the proton gradient builds up, and it resumes at once when an uncoupler is added.
The other three are true electron transport inhibitors: rotenone blocks Complex I (NADH to ubiquinone), antimycin A blocks Complex III at the Qi site, and cyanide binds the haem a3-CuB centre of cytochrome c oxidase (Complex IV).
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