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Discussion

Moist heat kills microorganisms principally by

  • A.oxidation of the constituents of the cytoplasm
  • B.alkylation of nucleic acid bases
  • C.dissolution of membrane lipids
  • D.irreversible coagulation and denaturation of proteins

Answer: D

Saturated steam denatures enzymes and structural proteins beyond recovery, and the presence of water markedly lowers the temperature at which that coagulation occurs. Dry heat, lacking water, has to work by oxidation and therefore needs far higher temperatures.

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