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Tyndallization achieves its effect by

  • A.alternating heating with freezing to rupture cells
  • B.heating in the presence of a chemical sporicide
  • C.repeated heating on successive days to catch germinating spores
  • D.a single very long exposure to saturated steam under raised pressure

Answer: C

Heating on one day kills vegetative cells, surviving spores germinate overnight, and the next day's heating kills the new vegetative cells; three cycles usually suffice. It was devised for materials that cannot tolerate autoclave temperatures.

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