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Bacteria are removed from a heat-labile solution by membrane filtration using a pore size of about

  • A.0.22 micrometres
  • B.2.2 micrometres, ten times wider
  • C.22 micrometres
  • D.220 micrometres

Answer: A

A 0.22 micrometre membrane retains vegetative bacteria while letting the fluid through, which is how sera, vaccines and antibiotic solutions are handled. Note that it does not reliably retain viruses or mycoplasmas, so a filtered solution is not necessarily sterile.

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