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Lyophilization preserves cultures by

  • A.heating gently in the presence of glycerol
  • B.suspending the organisms in strong salt solution
  • C.exposing them briefly to ultraviolet light
  • D.freezing and then drying under vacuum

Answer: D

Freeze-drying removes water by sublimation from the frozen state, leaving organisms viable but metabolically inert for years. It preserves rather than kills, which is why it is a storage method and not a sterilization method.

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