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Prions are of particular concern in sterilization because they

  • A.are killed only by prolonged refrigeration
  • B.are removed easily by ordinary membrane filtration
  • C.cannot survive outside living tissue at all
  • D.resist conventional autoclave cycles and many disinfectants

Answer: D

Having no nucleic acid and being an unusually stable misfolded protein, a prion survives standard cycles and routine chemicals, so special extended cycles and strong sodium hydroxide or hypochlorite are recommended. They sit beyond spores at the top of the resistance scale.

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