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Membrane filtration does not guarantee a sterile product because

  • A.the process requires heating the fluid
  • B.viruses and mycoplasmas can pass the pores
  • C.the filter itself adds contaminating organisms
  • D.bacteria multiply within the membrane

Answer: B

A 0.22 micrometre pore retains vegetative bacteria but is wide open to anything smaller, so a filtered solution is bacteria-free rather than sterile. Removing viruses needs a much finer membrane or a different method altogether.

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