Discussion
Match Group-I (enzyme), Group-II (reaction catalyzed by the enzyme), and Group-III (subcellular localization of the enzyme and the associated metabolic process) in CORRECT combination.
| Group-I | Group-II | Group-III |
|---|---|---|
| P. PEP Carboxylase | I. 2 Glycolate + 2 O2 = 2 Glyoxylate + 2 H2O2 | a. Cytosol - C4 cycle |
| Q. Rubisco | II. Pyruvate + NAD+ + CoA = Acetyl-CoA + CO2 + NADH | b. Peroxisome - C2 cycle |
| R. Glycolate oxidase | III. Phosphoenolpyruvate + HCO3− = Oxaloacetate + Pi | c. Mitochondria - aerobic respiration |
| S. Pyruvate dehydrogenase | IV. 3 (Ribulose 1,5-bisphosphate) + 3 CO2 + 3 H2O = 6 (3-phosphoglycerate) + 6 H+ | d. Chloroplast - C3 cycle |
Answer: A
The correct combination is P-III-a, Q-IV-d, R-I-b, S-II-c.
- PEP carboxylase fixes HCO3- onto phosphoenolpyruvate to give oxaloacetate (III), in the mesophyll cytosol, the first step of the C4 cycle (a).
- Rubisco carboxylates ribulose 1,5-bisphosphate to two molecules of 3-phosphoglycerate (IV), in the chloroplast stroma, in the C3 (Calvin) cycle (d).
- Glycolate oxidase oxidises glycolate to glyoxylate with O2, producing H2O2 (I), in the peroxisome, as part of photorespiration, the C2 cycle (b).
- Pyruvate dehydrogenase converts pyruvate, NAD+ and CoA to acetyl-CoA, CO2 and NADH (II), in the mitochondrial matrix, linking glycolysis to aerobic respiration (c).
Options B, C and D attach the wrong reaction to PEP carboxylase, Rubisco or pyruvate dehydrogenase.
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