Discussion
The PCR technique that allows measuring of DNA amplification at each cycle of PCR rather than the end point detection is :
Answer: C
The technique is Real Time PCR.
Real time (quantitative) PCR measures the product as it accumulates, cycle by cycle, through a fluorescent signal from a DNA-binding dye or a sequence-specific probe. The cycle at which fluorescence crosses a threshold reflects the starting amount of template.
Hot start PCR keeps the polymerase inactive until the first heating step to prevent non-specific priming. Nested PCR uses a second primer pair inside the first product to improve specificity. Reverse transcriptase PCR first copies RNA into cDNA. All three are normally read at the end point, on a gel.
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