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Which of the following technology is used in detection of viral plant pathogens ?

  • A.ELISA
  • B.Hyperspectral imaging
  • C.PCR
  • D.All of these

Answer: D

The answer is All of these.

ELISA (enzyme-linked immunosorbent assay) detects the virus's coat protein with specific antibodies and is the routine test for indexing planting material. PCR, or RT-PCR for RNA viruses, detects the viral nucleic acid and is more sensitive. Hyperspectral imaging records reflectance in many narrow bands and picks up changes in leaf pigments and structure caused by infection, which allows non-destructive detection over a field, often before symptoms are visible.

Since all three are used, choosing any one alone is incomplete.

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