Discussion
Why does high absorbance become non-linear before that limit is reached?
Answer: A
As the transmitted light falls towards the stray light level, the two become comparable and the measured transmittance is dominated by light the sample never absorbed, so the curve bends towards the concentration axis well before the theoretical limit. Detector response is linear in intensity, fluorescence is a separate problem, and drift is corrected by a double-beam arrangement. A floor set by an additive error always compresses readings that approach it, whatever the measurement.
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