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What does a double-beam instrument compare?

  • A.Two detectors measuring the same beam
  • B.The sample before and after a chemical reaction
  • C.Sample and reference at the same instant
  • D.Two different wavelengths through one sample

Answer: C

A double-beam arrangement splits the light and passes half through the sample and half through a reference, comparing them continuously, so drift in the source or the detector affects both paths equally and cancels. Wavelength comparison, dual detectors and time comparison describe other techniques. Measuring a ratio rather than an absolute value is what makes an instrument immune to the drift of everything common to the two paths. A single measurement chosen to respond to the one asymmetry beats several that respond to shared behaviour.

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