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What does a blank correct for?

  • A.Absorbance from the solvent, cell and reagents
  • B.Drift in the source lamp over time
  • C.Non-linearity at high absorbance readings
  • D.Stray light within the monochromator

Answer: A

A blank contains everything the sample does except the analyte, so subtracting its reading removes the contribution of solvent, cell walls and reagents. Lamp drift is what a double beam corrects, and neither non-linearity nor stray light is addressed by a blank. Measuring what everything except the quantity of interest contributes, and subtracting it, is the most general technique in analytical measurement and appears under many names. Most errors here come from carrying a result across from a neighbouring case where it does not hold.

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