Discussion
Why must sample and reference cells be matched?
Answer: B
Any difference in wall thickness, glass quality or surface finish appears in the comparison as an apparent absorbance that has nothing to do with the sample. Path length does matter but through the Beer-Lambert law rather than the wavelength, focus is unaffected, and volume is irrelevant to the measurement. Cells are sold in matched sets and marked so they are always inserted the same way round, which is a discipline worth keeping. Reading the definition literally, rather than recalling a formula, is the reliable route through questions of this shape.
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