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At roughly what absorbance is photometric error least?

  • A.Around 2.5
  • B.Around 0.4
  • C.Around 0.05
  • D.Around 1.5

Answer: B

The relative error curve has a broad minimum near an absorbance of about 0.4, where a given uncertainty in transmittance translates into the smallest relative uncertainty in concentration. Very low absorbance means a small difference between two large numbers, and very high absorbance means measuring a tiny transmitted signal. Working in the range from about 0.2 to 0.8 is standard practice, and diluting or changing the cell length is how a reading is brought into it.

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