Discussion
A calibration line does not pass through the origin. What should be suspected?
Answer: B
An intercept means something absorbs even at zero analyte, so the blank is not accounting for everything the standards contain. Varying absorptivity and stray light bend the line rather than shifting it, and a wrong path length changes the slope. Whether a defect changes a line's slope, its intercept or its straightness distinguishes three quite different causes, and that is the first question to ask of a calibration that looks wrong. Framing the question as which quantity the change acts upon makes the answer follow without calculation.
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