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Readings drift steadily upward during a long run on a single-beam instrument. Why?

  • A.The detector is saturating at high light levels
  • B.The monochromator's wavelength setting has shifted
  • C.The source lamp is warming or ageing
  • D.The sample is reacting with the solvent

Answer: C

With one beam any change in source output or detector response appears directly in the reading, and a lamp's output drifts as it warms and over its life. A reacting sample is possible but would affect only that sample, saturation does not drift, and a wavelength shift would be abrupt. The remedy is to re-zero on the blank frequently, or to use a double-beam instrument where the drift cancels in the ratio.

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