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What is an isosbestic point?

  • A.The point at which a titration reaches equivalence
  • B.The absorbance at which photometric error is least
  • C.A wavelength where two forms absorb equally
  • D.The wavelength of maximum absorbance for a compound

Answer: C

When a substance exists in two interconverting forms, there is generally a wavelength at which both have the same absorptivity, so the total absorbance there is independent of how the two are proportioned. It is neither a maximum, an equivalence point nor an error minimum. Measuring at such a point gives the total concentration regardless of the equilibrium, which is a useful trick when the ratio of forms cannot be controlled. A limit that survives making every external component ideal is an intrinsic one, and worth knowing for that reason.

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