Discussion
Consider the following statements about the Beer-Lambert law.
1. Absorbance is proportional to concentration
2. Absorbance is proportional to path length
3. Transmittance is proportional to concentration
Which of the statements given above is or are correct?
Answer: B
The first two are correct and the third is not: transmittance falls exponentially with concentration, which is precisely why absorbance is defined as its negative logarithm. Confusing the two is the commonest error in this topic and it produces a calibration that curves when it should be straight. A quantity defined specifically to linearise a relationship should not be interchanged with the one it replaced. Reading the definition literally, rather than recalling a formula, is the reliable route through questions of this shape.
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