Discussion
Consider the following pairs of instrument part and its function.
Monochromator - Selecting a narrow wavelength band
Sample cell - Defining the path length
Detector - Converting light into a signal
Blank - Correcting for stray light
How many of the pairs given above are correctly matched?
Answer: C
The first three are correctly paired and the fourth is not, so three match. A blank corrects for absorbance by the solvent, cell and reagents; stray light is an instrument defect that a blank does not remove, since it affects the blank and the sample differently. Attributing a correction to the wrong mechanism leads to trusting readings that the correction never actually fixed.
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