Discussion
Why is a reference cuvette still needed in a double-beam instrument?
Answer: B
Without a matched cell in the reference path the sample cell's walls and the solvent would appear as absorbance, since only one path would contain them. Beam intensity, detector balance and chopper operation are not the reason. The double-beam arrangement cancels whatever is common to the two paths, so anything that must cancel has to be placed in both.
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