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What does a spectrum plotted as absorbance against wavelength reveal?

  • A.The instrument's stray light performance
  • B.The path length of the cell used
  • C.The positions and shapes of absorption bands
  • D.The concentration of the analyte directly

Answer: C

A spectrum shows where the compound absorbs and how sharply, from which the analytical wavelength is chosen and the chromophores present may be inferred. Concentration follows only once the absorptivity is known, and neither stray light nor path length is displayed. Running a full spectrum before setting up a quantitative method is what identifies the best wavelength and warns of interfering absorption, and skipping it is how a method comes to be built on the wrong peak.

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