Discussion
Why is a spectrum run before a quantitative method is fixed?
Answer: D
The spectrum shows where the analyte absorbs most strongly and most flatly, and whether anything else in the sample absorbs there too. Resolution, dark current and refractive index are instrument or solvent properties measured separately. Choosing an analytical wavelength without looking at the spectrum first is choosing blind, and it is the step most often skipped when a published method is adapted to a new matrix.
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