Discussion
Secondary conformation of a globular protein in solution can be best determined by :
Answer: D
Secondary conformation is best determined by Circular dichroism.
Circular dichroism measures the different absorption of left- and right-circularly polarised light. In the far ultraviolet (about 190 to 250 nm) the peptide backbone gives characteristic spectra for alpha-helix, beta-sheet and random coil, so CD estimates the secondary structure of a protein in solution quickly and with little material.
Fluorescence spectroscopy reports on the surroundings of tryptophan and tyrosine side chains, mostly tertiary structure. Electron microscopy images particles but does not resolve secondary structure in solution, and analytical ultracentrifugation gives mass, shape and association state.
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