Discussion
Which of the following would NOT be a reason for a deviation from Beer's law?
Answer: C
Correct answer: using absorbance rather than transmittance as the measured quantity
Using absorbance is not a deviation but the correct practice, since it is absorbance that is linear in concentration. The other three are genuine causes of departure from Beer’s law: stray light sets a ceiling on the apparent absorbance, a polychromatic beam averages over absorptivities that differ across the band, and association or dissociation at high concentration changes the absorbing species itself.
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