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Sterilisation of the medium before a fermentation is necessary because

  • A.the medium would otherwise be too viscous
  • B.the pH would drift out of range
  • C.the vessel itself would corrode
  • D.a contaminant would outgrow the culture

Answer: D

Correct answer: a contaminant would outgrow the culture

A fermentation medium is a rich growth substrate at a comfortable temperature, so any organism entering it will grow - and a contaminant with a shorter generation time will overtake the production strain and consume the substrate for nothing. The whole vessel is then lost. Sterilising the medium, the air and the vessel is therefore a precondition rather than a precaution.

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