Discussion
Match the molecules in Column-I with their correct property/function in Column-II
| Column-I | Column-II |
|---|---|
| P. RNase P | 1. rRNA gene transcription |
| Q. RNA Polymerase-I | 2. Gene silencing |
| R. siRNA | 3. Cas9-mediated genome editing |
| S. Guide RNA | 4. Ribozymes |
| 5. tRNA gene transcription |
Answer: C
The correct match is P-4 ; Q-1 ; R-2 ; S-3.
- RNase P processes the 5' end of pre-tRNA, and its catalytic subunit is RNA, which makes it one of the classic ribozymes (4).
- RNA polymerase I transcribes the large rRNA genes in the nucleolus (1).
- siRNAs guide the RISC complex to complementary mRNA, causing its cleavage: gene silencing by RNA interference (2).
- A guide RNA directs the Cas9 nuclease to its DNA target in CRISPR-Cas9 genome editing (3).
Item 5, tRNA gene transcription, is the job of RNA polymerase III, the trap in the first option. The other options also give RNase P or siRNA roles they do not have.
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