Discussion
Which binding is inhibited by pactamycin?
Answer: B
Correct answer: Initiator-tRNA to 30S/40S initiation complexes
Pactamycin blocks initiation: it prevents the initiator tRNA from binding to the small subunit initiation complex, so no ribosome can start reading the message. Because it acts before elongation begins, ribosomes already engaged run to the end and come off - which is why the drug is used experimentally to clear the message of ribosomes.
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