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Discussion

Puromycin halts translation because it structurally resembles ________.

  • A.an aminoacyl-tRNA
  • B.a stop codon
  • C.the Shine-Dalgarno sequence
  • D.the sigma factor

Answer: C

Correct answer: an aminoacyl-tRNA

Puromycin mimics the aminoacyl end of a charged tRNA, so the ribosome transfers the growing chain onto it. The resulting peptidyl-puromycin cannot be translocated and falls off, releasing an incomplete polypeptide.

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