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Two compounds absorb at the same wavelength. How can both be determined?

  • A.Use a narrower slit to separate the bands
  • B.Measure at a single isosbestic point
  • C.Measure at two wavelengths and solve simultaneously
  • D.Dilute the sample until only one absorbs

Answer: C

Absorbances add, so measurements at two wavelengths give two equations in the two unknown concentrations, provided the absorptivity ratios differ between them. Dilution reduces both equally, a narrower slit cannot separate overlapping broad bands, and an isosbestic point gives a total rather than the individual values. Counting unknowns against independent measurements settles how much data any determination needs. A single measurement chosen to respond to the one asymmetry beats several that respond to shared behaviour.

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